Journal: Scientific Reports

Article Title: 3D culture of alginate-hyaluronic acid hydrogel supports the stemness of human mesenchymal stem cells

doi: 10.1038/s41598-024-54912-1

Figure Lengend Snippet: The cell proliferation and morphology of 3D hMSCs in biomaterial hydrogels. ( A ) The proliferation capacity of MSCs in various biomaterial hydrogel cultures for 15 days, as determined by the resazurin assay. ( B ) The proliferation rate of 2D and 3D AL-HA hydrogels during 15 days of culture and ( C ) The morphology of MSCs in 2D and 3D biomaterial hydrogels at day 7 of culture.

Article Snippet: The Umbilical cord-derived hMSCs (hUC-MSCs) was derived from I Wellness Co., Ltd. (Mueng, Nakhon Ratchasima, Thailand) by a healthy female donor aged 39 years who obtained written informed consent in accordance with the Human Research Ethics Committee under ethically acceptable terms of Suranaree University of Technology (EC63-06).

Techniques: Resazurin Assay

Journal: Scientific Reports

Article Title: 3D culture of alginate-hyaluronic acid hydrogel supports the stemness of human mesenchymal stem cells

doi: 10.1038/s41598-024-54912-1

Figure Lengend Snippet: The characterization of hMSCs. The expression of MSCs surface markers. Representative flow cytometry analyses for the expression of the positive markers, ( A ) Mouse IgG1 Anti-Human CD73-PeCY7, ( B ) Mouse IgG2A Anti-Human CD90-FITC and ( C ) Mouse IgG1 Anti-Human CD105-PE, the negative Markers, ( D ) Mouse IgG1 Anti-Human CD34-PE and ( E ) Mouse IgG1 Anti-Human CD45-PC5. ( F ) Trilineage differentiation of hMSCs: osteogenic, chondrogenic, and adipogenic differentiation.

Article Snippet: The Umbilical cord-derived hMSCs (hUC-MSCs) was derived from I Wellness Co., Ltd. (Mueng, Nakhon Ratchasima, Thailand) by a healthy female donor aged 39 years who obtained written informed consent in accordance with the Human Research Ethics Committee under ethically acceptable terms of Suranaree University of Technology (EC63-06).

Techniques: Expressing, Flow Cytometry

Journal: Stem Cells Translational Medicine

Article Title: Single-Cell RNA-Seq Reveals LRRC75A -Expressing Cell Population Involved in VEGF Secretion of Multipotent Mesenchymal Stromal/Stem Cells Under Ischemia

doi: 10.1093/stcltm/szad029

Figure Lengend Snippet: Effects of ischemia on VEGF secretion in 11 bone marrow (BM)-multipotent mesenchymal stromal/stem cells (MSCs) from different donors. ( A ) Schematic illustration of the protocol for culturing BM-MSCs under normoxic and ischemic conditions. ( B ) Variation in VEGF secretion among the 11 BM-MSC lines (L2, L3, L4, L5, L6, L7, L10, L11, L13, L14, and SB1) under normoxic and ischemic conditions. BM-MSCs were cultured under normoxic or ischemic conditions for 16 h. Level of VEGF secreted into the culture supernatant was determined using ELISA. The amount of VEGF secretion is given as the mean ± SEM ( n = 5 for each group). * P < .05; ** P < .01 (Student t -test).

Article Snippet: Human umbilical cord-derived MSCs (UC-MSCs) were obtained from PromoCell (Heidelberg, Germany) (C-12971), ScienCell Research Laboratories (7530), and ATCC (PCS-500-010).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Stem Cells Translational Medicine

Article Title: Single-Cell RNA-Seq Reveals LRRC75A -Expressing Cell Population Involved in VEGF Secretion of Multipotent Mesenchymal Stromal/Stem Cells Under Ischemia

doi: 10.1093/stcltm/szad029

Figure Lengend Snippet: Single-cell transcriptome profiling of 11 bone marrow (BM)-multipotent mesenchymal stromal/stem cells (MSCs) lines from different donors. ( A ) Schematic illustration of single-cell transcriptome experiments. BM-MSCs under normoxic conditions were individually captured for barcoding using Chromium controller (10X Genomics), and barcoded cDNA libraries were sequenced using Illumina sequencer. Then, individual cells were grouped based on their transcriptome similarity through unsupervised clustering (UMAP). ( B ) Number of captured cells that passed quality control and the median number of detected genes per cell for each MSC line under normoxic conditions. ( C ) Visualization based on 2-dimensional UMAP plotted as UMAP1 ( x -axis) vs. UMAP2 ( y -axis) for each cell type of the 11 BM-MSC lines under normoxic conditions. Eight clusters (CL0-7) marked with different colors were obtained using Seurat’s graph-based clustering algorithm. ( D ) UMAP visualization for each of the 11 BM-MSC lines.

Article Snippet: Human umbilical cord-derived MSCs (UC-MSCs) were obtained from PromoCell (Heidelberg, Germany) (C-12971), ScienCell Research Laboratories (7530), and ATCC (PCS-500-010).

Techniques: Control

Journal: Stem Cells Translational Medicine

Article Title: Single-Cell RNA-Seq Reveals LRRC75A -Expressing Cell Population Involved in VEGF Secretion of Multipotent Mesenchymal Stromal/Stem Cells Under Ischemia

doi: 10.1093/stcltm/szad029

Figure Lengend Snippet: Silencing of LRRC75A expression affects VEGF secretion induced by ischemic treatment. ( A ) Expression of LRRC75A is represented using UMAP visualization (cell positions are from the UMAP plot in ). LRRC75A was more highly expressed in CL3 than in other clusters. ( B and C ) Bone marrow (BM)-multipotent mesenchymal stromal/stem cells (MSCs) were transfected with either control siRNA (siNegative) or LRRC75A siRNA (siLRRC75A#1) under normoxic and ischemic conditions. mRNA level for LRRC75A (B) and VEGF secretion level in the supernatant (C) were determined using qPCR and ELISA, respectively. ( D ) Fold change of VEGF secretion level under ischemic conditions relative to that under the normoxic conditions are represented based on the result shown in (C). All values indicate the mean ± SEM of 3 independent biological replicates. (* P < .05, ** P < .01, Student’s t -test).

Article Snippet: Human umbilical cord-derived MSCs (UC-MSCs) were obtained from PromoCell (Heidelberg, Germany) (C-12971), ScienCell Research Laboratories (7530), and ATCC (PCS-500-010).

Techniques: Expressing, Transfection, Control, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments

doi: 10.3389/fcell.2020.00327

Figure Lengend Snippet: (A) Representative fluorescence images of 3D kidney microtissues comprised of podocyte (green) and mesenchymal stem cells (MSCs; red) fabricated using a PANDA chip. The microtissues were formed within 24 h. (B) The diameters of the formed 3D microtissues after 1 day of culture (* P < 0.05; **** P < 0.001; N = 20). (C) The diameters of the formed 3D microtissues cultured on different days (**** P < 0.001. N = 20). (D) Representative fluorescence images of 3D kidney microtissues cultivated for various periods. (E) Confocal images of live (green) and dead (red) cells. All scale bars represent 200 μm.

Article Snippet: Human umbilical cord blood-derived MSCs that were transfected with red fluorescence protein (RFP) were obtained from the Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan ( ).

Techniques: Fluorescence, Cell Culture

Journal: Journal of Cellular and Molecular Medicine

Article Title: Unveiling exosomes: Cutting‐edge isolation techniques and their therapeutic potential

doi: 10.1111/jcmm.70139

Figure Lengend Snippet: Clinical applications of exosomes. Exosomes can be extracted from bodily fluids, lymph, bile, blood, urine and etc. Analysing the molecular contents of exosomes can provide unique opportunities in their identifications. Exosomes can be used as biomarkers in disease diagnosis and surveillance. Clinical treatment mostly involves these strategies: First, cargo, including drugs, nucleic acids and proteins, can be encapsulated in exosomes and targeted to the mentioned sites. Also, immunotherapy can be used in cancer and other disease therapies.

Article Snippet: Furthermore, the therapeutic use of exosomes derived from human umbilical cord MSCs has been approved to mitigate neuroinflammation and enhance therapeutic efficacy in AD‐affected mice by repairing cognitive dysfunctions and reducing amyloid beta protein (Aβ) deposition., Based on the promising results observed in these clinical trials, several biotechnology companies, including Celltex Therapeutics, are actively advancing the development of MSC‐derived exosomes (MSC‐Exo)‐based treatments specifically targeted at addressing Alzheimer's disease.

Techniques: Biomarker Discovery

Journal: Medical Oncology (Northwood, London, England)

Article Title: Engineered mesenchymal stem cells expressing CXCR4 and LIGHT enhance Anti-Tumor activity in a subcutaneous gastric cancer xenograft model

doi: 10.1007/s12032-026-03271-0

Figure Lengend Snippet: (a) Map of plasmid pEF1α-CXCR4-TNFSF14 showing the inserted CXCR4 and TNFSF14 genes with regulatory elements (b) Diagram showing HUC-MSCs engineered with lentiviral vectors carrying CXCR4 and TNFSF14 for tumor-targeted immunotherapy (c) Experimental timeline indicating the chronological sequence of procedures. Mice were first acclimatized for 7 days, followed by tumor cell inoculation (HGC-27) on Day 0. MSC treatments (unmodified or engineered Tg-HUC-MSCs) were administered on days 7, 11, and 15. Tumor growth was monitored using caliper measurements, and samples were collected at the end of the study on Day 34 for histological analysis

Article Snippet: Human umbilical cord-derived MSCs were gratis provided by Shenzhen Beike Biotechnology Co., Ltd.

Techniques: Plasmid Preparation, Sequencing

Journal: Medical Oncology (Northwood, London, England)

Article Title: Engineered mesenchymal stem cells expressing CXCR4 and LIGHT enhance Anti-Tumor activity in a subcutaneous gastric cancer xenograft model

doi: 10.1007/s12032-026-03271-0

Figure Lengend Snippet: Flow cytometry analysis of LIGHT and CXCR4 surface expression in engineered HUC-MSCs. Representative flow cytometry dot plots showing cell surface expression of LIGHT (CD258/TNFSF14) and CXCR4 (CD184) in control MSCs and Tg-MSCs transduced with increasing lentiviral doses (CT-5, CT-10, CT-20). GFP-positive cells indicate successfully transduced MSCs. Upper panels display LIGHT (CD258) expression, and lower panels display CXCR4 (CD184) expression. Progressive increases in the percentage of GFP⁺/CD258⁺ and GFP⁺/CD184⁺ cells were observed with higher viral doses, confirming dose-dependent and stable surface expression of both transgenes

Article Snippet: Human umbilical cord-derived MSCs were gratis provided by Shenzhen Beike Biotechnology Co., Ltd.

Techniques: Flow Cytometry, Expressing, Control, Transduction

Journal: Medical Oncology (Northwood, London, England)

Article Title: Engineered mesenchymal stem cells expressing CXCR4 and LIGHT enhance Anti-Tumor activity in a subcutaneous gastric cancer xenograft model

doi: 10.1007/s12032-026-03271-0

Figure Lengend Snippet: Luciferase-based cytotoxicity assay assessing the anti-tumor activity of Tg-MSCs against HGC-27 gastric cancer cells. HGC-27 cells stably expressing luciferase were co-cultured with unmodified MSCs or Tg-MSCs at effector-to-target ratios of 1:1, 5:1, and 10:1. Luciferase activity was measured as relative light units (RLU) and normalized to target-only controls (100% viability). Data are presented as percentage tumor cell killing derived from normalized luciferase signal (mean ± SD, n = 3). Tg-MSCs induced significantly higher cytotoxicity than unmodified MSCs at all ratios ( p < 0.001, one-way ANOVA with Tukey’s post-test)

Article Snippet: Human umbilical cord-derived MSCs were gratis provided by Shenzhen Beike Biotechnology Co., Ltd.

Techniques: Luciferase, Cytotoxicity Assay, Activity Assay, Stable Transfection, Expressing, Cell Culture, Derivative Assay

Journal: Medical Oncology (Northwood, London, England)

Article Title: Engineered mesenchymal stem cells expressing CXCR4 and LIGHT enhance Anti-Tumor activity in a subcutaneous gastric cancer xenograft model

doi: 10.1007/s12032-026-03271-0

Figure Lengend Snippet: Scratch wound migration assay showing the effect of MSCs and Tg-MSCs on HGC-27 gastric cancer cell migration. Representative images were captured at 12 h and 24 h following scratch induction. Red dashed lines indicate wound boundaries. Tg-MSC treatment markedly delayed wound closure compared with control and MSC-treated groups, indicating inhibition of tumor cell migration

Article Snippet: Human umbilical cord-derived MSCs were gratis provided by Shenzhen Beike Biotechnology Co., Ltd.

Techniques: Migration, Control, Inhibition

Journal: Medical Oncology (Northwood, London, England)

Article Title: Engineered mesenchymal stem cells expressing CXCR4 and LIGHT enhance Anti-Tumor activity in a subcutaneous gastric cancer xenograft model

doi: 10.1007/s12032-026-03271-0

Figure Lengend Snippet: (a) Representative tumor measurements across groups, confirming consistent tumor suppression by Tg-MSCs (b) Tumor growth curves showing significantly reduced tumor volumes in Tg-MSC–treated mice compared to controls and MSCs ( p < 0.001) before and after taking out the outliers (c) Body weight changes in control, Ctrl-MSC, and Tg-MSC groups. No significant differences were observed, indicating that Tg-MSCs did not cause systemic toxicity. (d) Ki-67 immunohistochemistry of tumor xenografts from PBS-treated control, MSC-treated, and Tg–MSC–treated groups. Tumors from control animals exhibited extensive nuclear Ki-67 staining, consistent with high proliferative activity. MSC-treated tumors showed reduced and more heterogeneous Ki-67 expression. Tg-MSC–treated tumors demonstrated markedly diminished Ki-67 positivity, with large regions lacking nuclear staining. Scale bars: 200 µM (e) Bar graph showing the percentage of Ki-67–positive proliferating cells in tumor tissues from the control, MSC-treated, and Tg-MSC–treated groups. Data are presented as mean ± SD. Tg-MSC treatment resulted in a significant reduction in Ki-67–positive cells compared with unmodified MSCs, indicating decreased tumor cell proliferation. Statistical significance was determined using one-way ANOVA (p< 0.01) (f) Representative H&E-stained sections of the heart, kidney, liver, lung, and spleen across the three treatment groups. All organs displayed preserved tissue architecture with no histopathological abnormalities observed. Scale bars: 100 µM

Article Snippet: Human umbilical cord-derived MSCs were gratis provided by Shenzhen Beike Biotechnology Co., Ltd.

Techniques: Control, Immunohistochemistry, Staining, Activity Assay, Expressing